Biochemistry

Browse the top 1% of biochemistry preprints on bioRxiv, ranked by QED Score for originality and validity. This collection features new research on protein structure and function, enzyme mechanisms, metabolism, and molecular interactions, with a claim-by-claim breakdown of each paper's evidence.

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QED Score is a validated AI-based quality metric that evaluates life science papers on two dimensions: originality and validity. Each manuscript is anonymized, broken down into its claims and supporting evidence, and examined by specialized AI agents for statistical rigor, consistency across figures, and fit with the existing literature. Scores are percentile ranks, so they're comparable across fields. In blinded tests, expert professors sided with QED Score over journal rank in 75% of decisive cases.

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Biochemistry

Glucose is a ligand-like trigger for secretion of postprandial GLP-1

De novo design of phosphotyrosine peptide binders

Coupling high-throughput protease enzymology with viral replication reveals biochemical constraints of viral fitness

Atypical GPCR Activation Resolved by Nanobody Engineering

Tryptophan Chemistry Driven by a Widespread Cytochrome P422 Enzyme Family

Stereospecific GPG acylation by CLN8 drives BMP biosynthesis and its loss leads to Batten disease

PtdIns(3,5)P2 Is an Endogenous Ligand of STING in Innate Immune Signaling

Nicotine biosynthesis completed by cryptic activating glucosylation

Conformation-specific Antibody Deciphers K27-linked Ubiquitination in Chaperone-Mediated Proteostasis

CLN8 enables a non-canonical phospholipid synthesis pathway

The Wnt/APC destruction complex targets SREBP2 in a β-catenin-independent pathway to control cholesterol metabolism

Molecular mechanism of naturally-encoded signaling-bias at the complement anaphylatoxin receptors

Genetic variation of human G6PD impacts Red Blood Cell transfusion efficacy

Deep Red Blood Cell Proteome Defines the Band 3 N-Terminus Interactome as a Regulator of Hypoxic Adaptation via BLVRB-Dependent S-Nitroso Transfer

Computational design of metalloproteases

Computational design of cysteine proteases

Nitrogen deletion in HDAC-targeting anticancer drug biosynthesis

Nanobody-Driven Stabilization Synergistically Rescues F508del-CFTR and Reveals an Alternative Active State of the Channel

De novo design of RNA and nucleoprotein complexes

A biased allosteric modulator functions as a molecular glue to induce β2AR dimerization

A Cryptic Interfacial Pocket Uncovered in Full CRL4CRBN–IKZF3 Ubiquitylation Complex Enhances IMiD Efficacy

Butyrolactol A potentiates caspofungin efficacy against resistant fungi via phospholipid flippase inhibition

NAC controls nascent chain fate through tunnel sensing and chaperone action

A small molecule inhibitor of NVL suppresses tumor growth by blocking ribosome biogenesis

Dual E3 ligase recruitment by monovalent degraders enables redundant and tuneable degradation of SMARCA2/4

NAC promotes co-translational protein folding at the ribosomal tunnel exit

Small molecule inhibitors of the NorA multidrug efflux pump potentiate antibiotic activity by binding the outward-open conformation

ACAD10 encodes two orphan enzymes in the ether lipid biosynthetic and salvage pathways

Allosteric Disordering of eIF2B Regulates the Integrated Stress Response

High-throughput diversification of protein-ligand surfaces to discover chemical inducers of proximity

Structure and substrate recognition by the Twin-arginine translocation (Tat) pathway core complex

ZNFX1 uses two-component ubiquitin circuitry to quarantine viral RNA

“Design principles of a membrane-spanning ubiquitin ligase”

A Chemoproteomic Atlas of the Human Purine Interactome for Regioselective Ligand Discovery

A biased allosteric modulator functions as a molecular glue to induce β2AR dimerization

De novo design of RNA and nucleoprotein complexes

Nanobody-Driven Stabilization Synergistically Rescues F508del-CFTR and Reveals an Alternative Active State of the Channel

Nitrogen deletion in HDAC-targeting anticancer drug biosynthesis

Computational design of cysteine proteases

Computational design of metalloproteases

De novo design of RNA and nucleoprotein complexes

“Design principles of a membrane-spanning ubiquitin ligase”

Dual E3 ligase recruitment by monovalent degraders enables redundant and tuneable degradation of SMARCA2/4

Genetic variation of human G6PD impacts Red Blood Cell transfusion efficacy

Glucose is a ligand-like trigger for secretion of postprandial GLP-1

Hierarchical Anchoring–Gating Dictates Specific H4K16 Acetylation by the Human MSL Complex

High-throughput biochemical phenotyping of SHP2 variants reveals the molecular basis of diseases and allosteric drug inhibition

High-throughput diversification of protein-ligand surfaces to discover chemical inducers of proximity

In vivo-compatible spatial multi-omics via hydrogen peroxide-independent APEX2 labeling

Mapping the mammalian dark metabolome by in vivo isotope tracing

MDA5 multimerization on LINE RNA drives pathogenic extracellular immune complexes in autoimmunity

Mechanistic machine learning enables interpretable and generalizable prediction of prime editing outcomes

Molecular dissection of protein complexes isolated from sections of human brain

Molecular mechanism of naturally-encoded signaling-bias at the complement anaphylatoxin receptors

NAC controls nascent chain fate through tunnel sensing and chaperone action

NAC promotes co-translational protein folding at the ribosomal tunnel exit

Nanobody-Driven Stabilization Synergistically Rescues F508del-CFTR and Reveals an Alternative Active State of the Channel

Nicotine biosynthesis completed by cryptic activating glucosylation

Nitrogen deletion in HDAC-targeting anticancer drug biosynthesis

Pan-repository analysis reveals a drug-activating function of microbial bile acid conjugation

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